Minimum quantity Discount
Buy 2 + Get 10% discount
Skip to content

a-Chymotrypsin from bovine pancreas Type II, lyophilized powder, =40 units/mg protein

Regular price $101.25

SKU: C4129-250MG

a-Chymotrypsin from bovine pancreas Type II, lyophilized powder, =40 units/mg protein

CAS Number 9004-07-3
EC Number 232-671-2
Enzyme Commission (EC) Number ( BRENDA | IUBMB )
MDL number MFCD00130481

Analysis Note
Protein determined by E1%/280

Biochem/physiol Actions
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.

Preparation Note
Derived from New Zealand-sourced pancreas

Produced from 3× crystallized chymotrypsinogen

Unit Definition
One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).

Quality Level PREMIUM
type Type II
form lyophilized powder
mol wt mol wt 25 kDa
composition protein, ≥85%
color white to off-white
storage temp. −20°C
Gene Information cow ... CTRB1(618826)
Back to top

Added to cart