Vitrification Method Warming Kit
– Warming Solution (TS) :1 vial of 1.8mℓ
– Diluent Solution (DS) :1 vial of 0.5mℓ
– Washing Solution (WS) :1 vial of 1.0mℓ
– 1 Warming Plate with 4 wells
ECMPC - Warming Solution Set
SKU: ECMPC-220
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ECMPC Warming Solution Set for Human Embryo and Oocyte Vitrification
The ECMPC Warming Solution Set, developed by the renowned vitrification pioneer Dr. Masashige Kuwayama, offers a superior protocol for the safe and effective warming of human embryos and oocytes after vitrification. Designed with cutting-edge technology and backed by over 15 years of experience, ECMPC’s warming media ensures optimal recovery rates, providing security and reliability in reproductive procedures worldwide.
Why Choose ECMPC Warming Solution Set?
• Developed by Dr. Masashige Kuwayama: Created by the expert who pioneered vitrification media, this solution set is based on extensive research and practical success.
• Proven Effectiveness: ECMPC’s warming media is part of a globally trusted protocol for high survival rates in oocyte and embryo cryopreservation.
• FDA 510(k) Cleared: Certified for use in clinical settings, ensuring safety and efficacy.
• Advanced Technology: Our warming protocol is based on the latest scientific advancements, offering effectiveness and security to every user.
Warming Solution Kit Components:
• Warming Solution (TS): 10 vials (1.8 mℓ)
• Diluent Solution (DS): 2 vial (0.5 mℓ)
• Washing Solution (WS): 4 vial (1.0 mℓ)
Additional tools required: Microscope, Stopwatch (count-up function), Tweezers, Micropipette (300μℓ), Warming Plate with 4 wells
SHIPPING: Overnight Shipping Required to keep media at temperature - 2-8 degrees celsius.
MATERIALS
- Microscope (Turn off the heating plate)
- Stop watch (With count up function)
- Tweezers
- Micro pipette for 300μℓ
WARMING (1 Minute)
See Image Below
1.- Take the TS vial out of the incubator, and expel all of the solution out of it into the TS well (1.8ml, Fig. 8, Step1/①)
2.- Quickly (within 1 sec) put the Vitrification method from liquid nitrogen into the TS well (Fig. 8, Step1/②). Start counting up by the stop watch for 1 min.
3.- The oocyte/embryo releases from the Vitrification method sheet by itself, and begins to float.
VITRIFICATION1 (30 - 40 SECONDS)
1.- Aspirate the oocyte/embryo first, followed by 3mm of the TS into the pipette (Fig. 9， 1).
2.- Introduce the TS to the bottom of the DS well (Fig. 9, 2), then expel the oocyte/embryo slowly to the bottom of TS layer in DS well (Fig. 9, 3), and wait for 3 min (Fig. 8, Step 2/①).
3.- While waiting, fill the WS1 and the WS2 well with 300μℓ each of ws Solution (Fig. 8, Step 3/①).
STEP 1 STEP 2 STEP 3
WASHING (5 MINUTES)
1.- Aspirate the oocyte/embryo followed by 3mm of the DS into the pipette (Fig. 10，1).
2.- Introduce the DS to the bottom of the WS1 (Fig. 10, 2), and expel the oocyte/embryo slowly to the bottom of the DS layer in WS1 well (Fig. 10.3). Observe the shape of the oocyte/embryo and memorize it. Turn off the light, and wait for more than 3 min.
3.- After 3 min, compare the shape of the oocyte/embryo to the one memorized. Give ａ survival judgment if the shrinkage of the oocyte is recovered.
4.- Wait for 5 min in total (Fig. 8, Step3/②).
WASHING 2 (1 MIN)
1.- Aspirate the oocyte/embryo with minimal volume of the WS1.
2.- Put the oocyte/embryo on the surface of the WS2 well (Fig. 8, Step3/③).
3.- After the oocyte/embryo sinks to the bottom, aspirate and place it on the surface of a different location with in W2. Put the oocyte/embryo into the droplet of the culture media until ICSI or ET is performed.
4.- (Two to four hours culture for ICSI, and 3 hours for blastocyst transfer are recommended)
This media is now 510K Cleared.
