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ZyMōt Multi 850µl device recovers sperm with superior genomic integrity

We are very excited to share this study which documents how the ZyMōt Multi 850µl device recovers sperm with superior genomic integrity to improve chances of obtaining a euploid conceptus for ICSI patients who have experienced recurrent miscarriages.  

A treatment approach for couples with disrupted sperm DNA integrity and recurrent ART failure [Full Article]

Alessandra Parrella & Derek Keating & Stephanie Cheung & Philip Xie & Joshua D. Stewart & Zev Rosenwaks & Gianpiero D. Palermo

Journal of Assisted Reproduction and Genetics
https://doi.org/10.1007/s10815-019-01543-5
Received: 3 May 2019 / Accepted: 23 July 2019

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Abstract

Objective
To test a novel method to select spermatozoa with high chromatin integrity.

Design
Specimens with high sperm chromatin fragmentation (SCF) were selected by density gradient selection (DGS) and microfluidic sperm sorting (MSS).
Setting
Academic medical center.

Patient(s)
Ejaculates from consenting men were processed by DGS/MSS. Couples underwent ICSI cycles with spermatozoa processed by DGS/MSS. Clinical outcomes were evaluated after embryo transfer.

Intervention(s)
SCF was measured by TUNEL. ICSI with spermatozoa selected by DGS and MSS
was performed.

Main outcome measure(s) Fertilization, embryo implantation, and pregnancy outcomes were compared between DGS and MSS.

Result(s) A total of 23 men had an average SCF of 20.7 ± 10%. After DGS and MSS, the SCF was 12.5 ± 5% and 1.8 ± 1%, respectively. In couples who underwent ICSI, the average SCF was 28.8 ± 9%, which fell to 21.0 ± 9% after DGS and 1.3 ± 0.7% after MSS. Four couples underwent 11 ICSI cycles with DGS and achieved one (25%) pregnancy that resulted in pregnancy loss. In four subsequent ICSI cycles with MSS, an ongoing clinical pregnancy rate of 50% was achieved. Five additional couples underwent 12 cycles of ICSI with DGS. After preimplantation genetic testing for aneuploidy, 30.3% of the embryos were euploid. One pregnancy was achieved, resulting in pregnancy loss. With MSS, 31.5% of the embryos were euploid and 4 couples obtained a pregnancy. Finally, sixteen couples underwent 20 ICSI cycles solely with MSS at our center. Of these couples, 8 had failed 13 ICSI cycles with DGS elsewhere. These couples achieved an overall implantation of 34.5% (10/29) and a pregnancy rate of 58.8% (10/17).

Conclusion(s)
Microfluidic selection yielded spermatozoa with optimal genomic integrity and improved chances of obtaining a euploid conceptus.

Keywords
Assisted reproductive technologies . Sperm chromatin fragmentation . Density gradient selection . Microfluidic chamber . ICSI

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