Maintaining environmental stability inside the
incubator chamber is critical…

“…to reduce environmental stressors and to maintain appropriate growth conditions. This makes the laboratory incubator one of the most important pieces of equipment within the IVF laboratory and highlights the importance of incubator selection, which is one of the key decisions within the
IVF laboratory.”¹

Jason Swain, PhD, HCLD

^{1. Swain JE. Decisions for the IVF laboratory: comparative analysis of embryo culture incubators. Reprod Biomed Online. 2014;28(5):535-547.}

A history of trusted performance

^{2. Mortimer D, Jansen R, Henman MJ. Development of an Improved Embryo Cultures System for Clinical Human IVF. Brisbane, Australia; Cook Australia; 2002.}

Next-generation humidified incubation

Clinical evidence: humidified incubation and its impact on osmolality

+Toward a predictive theoretical model for osmolality rise with non-humidified incubation:
a randomized, multivariate response-surface study. (Mullen SF)

+Factors of the human embryo culture system that may affect media
evaporation and osmolality. (Mestres E, García-Jiménez M, Casals A, et al.)

+Impact of the type of incubator (non-humidified versus humidified) on embryo culture media osmolality. (Boumerdassi Y, Huet S, Millin M, et al.)

+Humidity and osmolality: Can we avoid media osmolality shifts in a dry environment? (Mullen SF)

+Controversies in ART: considerations and risks for uninterrupted embryo culture. (Swain JE)

+Unstable osmolality of microdrops cultured in non-humidified incubators. (Yumoto K, Iwata K, Sugishima M, et al.)

+Humidification of a dry benchtop IVF incubator: impact on culture media parameters. (Holmes R, Swain JE)

+Different mineral oils used for embryo culture microdrop overlay differentially impact media evaporation. (Swain JE)

+Media evaporation in a dry culture incubator; effect of dish, drop size and oil on media osmolality. (Swain JE, Graham C, Kile R, et al.)

+Media osmolality changes over 7 days following culture in a non-humidified benchtop incubator. (Swain J, Schoolcraft WB, Bossert N, et al.)

+Unstable osmotic pressure in microdrops cultured under mineral oil in non-humidified incubators. (Iwata K, Yumoto K, Mio Y)

+Microdrop preparation factors influence culture-media osmolality, which can impair mouse
embryo preimplantation development. (Swain JE, Cabrera L, Xu X,  et al.)

Clinical evidence: embryo culturing and humidified incubation

+Comparison of humidified versus non-humidified incubation with sequential culture media in
a time-lapse incubator using sibling oocytes splits. (Holmes R, Weinberg K, Kalaghan L, et al.)

+Humid vs. dry embryo culture conditions on embryo development: a continuous embryo
monitoring assessment. (Del Gallego R, Albert C, Marcos J, et al.)

+Humid versus dry incubator: a prospective, randomized, controlled trial. (Fawzy M, AbdelRahman MY, Zidan MH, et al.)

+Incorporation of the Cook K-MINC incubator and media system into the IVF lab: the future of IVF. (Lee M, Grazi R, Seifer DB)

+Cell volume regulation in oocytes and early embryos: connecting physiology to successful culture media. (Baltz JM, Tartia AP)