{"product_id":"sigma-mineral-oil-light-oil-neat-bioreagent-suitable-for-mouse-embryo-cell-culture","title":"SIGMA Mineral oil, light oil (neat), BioReagent, suitable for mouse embryo cell culture","description":"\u003ch2 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003eSIGMA Mineral oil, light oil (neat), BioReagent, suitable for mouse embryo cell culture\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eCAS No.:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e8042-47-5\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDensity:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e0.84g\/mLat 25°C(lit.)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eRecommended for overlaying mouse embryo cultures to prevent drying.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAnalysis Note:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eThis material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCaution:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eThis product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePackaging:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e5, 100, 500 mL in poly bottle\u003c\/p\u003e","brand":"Millipore Sigma","offers":[{"title":"1 EA \/ 5mL","offer_id":48398523433198,"sku":"M8410-5ML","price":34.0,"currency_code":"USD","in_stock":true},{"title":"1 EA \/ 100mL","offer_id":48398523465966,"sku":"M8410-100ML","price":59.0,"currency_code":"USD","in_stock":true},{"title":"1 EA \/ 500mL","offer_id":48398523498734,"sku":"M8410-500ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1 EA \/ 1 Liter","offer_id":48398523531502,"sku":"M8410-1L","price":168.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/2245\/7875\/files\/M8410_1L.avif?v=1785351034","url":"https:\/\/us.ivfstore.com\/products\/sigma-mineral-oil-light-oil-neat-bioreagent-suitable-for-mouse-embryo-cell-culture","provider":"IVF Store","version":"1.0","type":"link"}